Istnofret was the second ranked wife of Ramesses II and had several sons and daughters. She gave birth to Merneptah, Ramesses' heir, Prince Khaemweset, and Bint-Anath along with others. Istnofret replaced Queen Nefertari at some point after the dedication of the temples at Abu Simbel. What happened to Nefertari is not known for sure, she may have died or retired. Istnofret disappeared from record not long after. She may have died or retired to the harem enclave near the Fayoum region.